Composite

Part:BBa_K4011019:Design

Designed by: Xiaochong Ma   Group: iGEM21_LINKS_China   (2021-10-21)


pTALEsp2-TnaA-FL-FMO-B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1513
    Illegal NheI site found at 1921
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 113
    Illegal BamHI site found at 5978
    Illegal XhoI site found at 2152
    Illegal XhoI site found at 2692
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 747
    Illegal AgeI site found at 849
    Illegal AgeI site found at 1767
    Illegal AgeI site found at 2277
    Illegal AgeI site found at 4385
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

1. All codons were optimized for E. coli based on E. coli codon bias.

2. The sequences for the flexible linker is GGGGSGGGGS.

3. Transformed and expressed in E. coli DH5α ΔTnaA to negate influence of endogenous TnaA in measurements.


Source

Tryptophanase (TnaA) is from E. coli and flavin-containing monooxygenase (FMO) is from Methylophaga aminisulfidivorans.

References